pTargetAmp
(Plasmid
#228552)
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PurposeTemplate for PCR amplification for gRNA cloning plasmid by in vivo cloning.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTargetF
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Vector typeCRISPR
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAmpicillin resistance
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTargetAmp was a gift from Jeferson Gross (Addgene plasmid # 228552 ; http://n2t.net/addgene:228552 ; RRID:Addgene_228552)