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Addgene

pcDNA3.1-myc:Rbx1:gs10:FRB:HA
(Plasmid #228534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myc:Rbx1:gs10:FRB:HA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    696
  • Entrez Gene
    RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-myc:Rbx1:gs10:FRB:HA was a gift from Roman Jerala (Addgene plasmid # 228534 ; http://n2t.net/addgene:228534 ; RRID:Addgene_228534)

  • For your References section:

    Subunits of an E3 Ligase Complex as Degrons for Efficient Degradation of Cytosolic, Nuclear, and Membrane Proteins. Verbic A, Lebar T, Praznik A, Jerala R. ACS Synth Biol. 2024 Mar 15;13(3):792-803. doi: 10.1021/acssynbio.3c00588. Epub 2024 Feb 26. 10.1021/acssynbio.3c00588 PubMed 38404221
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