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FUGW-RUSH-Strep-KDEL
(Plasmid #228527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUWG
  • Backbone manufacturer
    FUGW was a kind gift from David Baltimore (Plasmid #14883)
  • Backbone size w/o insert (bp) 9955
  • Total vector size (bp) 10726
  • Modifications to backbone
    Chimeric Intron - IRES2 was introduced
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Strep-KDEL
  • Promoter Ubc
  • Tag / Fusion Protein
    • Streptavidin-KDEL (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FUGW (Addgene plasmid # 14883) was digested with XbaI and EcoRI. Strep-KDEL-intron-IRES was amplified from Str-KDEL_SBP-EGFP-Ecadherin (Addgene plasmid # 65286) with XbaI and EcoRI sites introduced and inserted into restricted FUGW by GIBSON assembly, creating FUGW-Strep-KDEL-intron-IRES (FUGW-RUSH for simplicity). FUGW-RUSH can be digested with EcoRI and BamHI to allow for downstream cloning of RUSH plasmids while maintaining 3' ATG on IRES2.

Please visit https://doi.org/10.1101/2024.05.16.594502 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-RUSH-Strep-KDEL was a gift from Ginny Farias (Addgene plasmid # 228527 ; http://n2t.net/addgene:228527 ; RRID:Addgene_228527)

  • For your References section:

    Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons. Li CH, Kersten N, Özkan N, Nguyen DTM, Koppers M, Post H, Altelaar M, Farias GG. Nat Commun. 2024 Dec;15: 10829. doi: 10.1038/s41467-024-55052-w. 10.1038/s41467-024-55052-w
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