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FUGW-RUSH-LAMP1-mScarleti
(Plasmid #228526)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228526 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUWG-RUSH-Strep-KDEL
  • Backbone size w/o insert (bp) 10726
  • Total vector size (bp) 12824
  • Modifications to backbone
    Chimeric Intron - IRES2 was introduced
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Promoter Ubc
  • Tags / Fusion Proteins
    • Streptavidin-KDEL (N terminal on backbone)
    • Streptavidin Binding Protein (SBP) (N terminal on insert)
    • mScarleti (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.16.594502 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-RUSH-LAMP1-mScarleti was a gift from Ginny Farias (Addgene plasmid # 228526 ; http://n2t.net/addgene:228526 ; RRID:Addgene_228526)

  • For your References section:

    Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons. Li CH, Kersten N, Özkan N, Nguyen DTM, Koppers M, Post H, Altelaar M, Farias GG. Nat Commun. 2024 Dec;15: 10829. doi: 10.1038/s41467-024-55052-w. 10.1038/s41467-024-55052-w
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