CROP-seq-mTurquoise2-v2-no-scaffold
(Plasmid
#228522)
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PurposeCROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCROPseq-puro-v2
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Vector typeLentiviral
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Selectable markersmTurquoise2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemTurquoise2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROP-seq-mTurquoise2-v2-no-scaffold was a gift from Jellert Gaublomme (Addgene plasmid # 228522 ; http://n2t.net/addgene:228522 ; RRID:Addgene_228522) -
For your References section:
Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap. Gu J, Iyer A, Wesley B, Taglialatela A, Leuzzi G, Hangai S, Decker A, Gu R, Klickstein N, Shuai Y, Jankovic K, Parker-Burns L, Jin Y, Zhang JY, Hong J, Niu X, Costa JA, Pezet MG, Chou J, Snoeck HW, Landau DA, Azizi E, Chan EM, Ciccia A, Gaublomme JT. Nat Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x. 10.1038/s41587-024-02386-x PubMed 39375448