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Addgene

Lact-C2-GFP
(Plasmid #22852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lactadherin-C2 Domain
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    475
  • Entrez Gene
    MFGE8
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lact-C2-GFP was a gift from Sergio Grinstein (Addgene plasmid # 22852 ; http://n2t.net/addgene:22852 ; RRID:Addgene_22852)

  • For your References section:

    Membrane phosphatidylserine regulates surface charge and protein localization. Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S. Science. 2008 Jan 11. 319(5860):210-3. 10.1126/science.1152066 PubMed 18187657
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