MG1655 tnaA-
(Bacterial strain
#228513)
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PurposeIndole-responsive Phage Infection Assay using LARP and T7 Phage with gp1 knock-out
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Bacterial Strain | 228513 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeThis is a strain, not a plasmid
Growth in Bacteria
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Bacterial Resistance(s)none
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Growth Temperature37°C
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Growth Strain(s)MG1655 tnaA-
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametnaA-
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Speciesn/a
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from E. coli K-12 MG1655 strain. This strain does not produce indole as measured with Kovac's Reagent Indole Test. A T7 phage strain with a gp1 KO is available from the Whitehead Lab.
Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MG1655 tnaA- was a gift from Timothy Whitehead (Addgene plasmid # 228513) -
For your References section:
Engineered Stop and Go T7 RNA Polymerases. Baumer ZT, Newton MS, Lofstrand L, Carpio Paucar GN, Farny NG, Whitehead TA. ACS Synth Biol. 2024 Dec 20;13(12):4165-4174. doi: 10.1021/acssynbio.4c00627. Epub 2024 Nov 28. 10.1021/acssynbio.4c00627 PubMed 39610115