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Addgene

US0 pyrF-/hisB-/tnaA-
(Bacterial strain #228512)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 228512 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Vector type
    This is a strain, not a plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    US0 pyrF-/hisB-/tnaA-
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pyrF-/hisB-/tnaA-
  • Species
    n/a

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from US0 pyrF-/hisB- (Scot Wolfe, Addgene #12614). This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. This strain is also has a deletion in tnaA gene and does not produce indole, verified by Kovac's Reagent.

Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    US0 pyrF-/hisB-/tnaA- was a gift from Timothy Whitehead (Addgene plasmid # 228512)

  • For your References section:

    Engineered Stop and Go T7 RNA Polymerases. Baumer ZT, Newton MS, Lofstrand L, Carpio Paucar GN, Farny NG, Whitehead TA. ACS Synth Biol. 2024 Dec 20;13(12):4165-4174. doi: 10.1021/acssynbio.4c00627. Epub 2024 Nov 28. 10.1021/acssynbio.4c00627 PubMed 39610115
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