pZB641_pT7_Lysin-KU1
(Plasmid
#228511)
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PurposeT7 RNAP inducible Lysin KU1 Expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepH3U3-Zif268
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Backbone manufacturerScot Wolfe, Addgene plasmid #12610
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLysin KU1
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SpeciesSynthetic
- Promoter T7
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZB641_pT7_Lysin-KU1 was a gift from Timothy Whitehead (Addgene plasmid # 228511 ; http://n2t.net/addgene:228511 ; RRID:Addgene_228511) -
For your References section:
Engineered Stop and Go T7 RNA Polymerases. Baumer ZT, Newton MS, Lofstrand L, Carpio Paucar GN, Farny NG, Whitehead TA. ACS Synth Biol. 2024 Dec 20;13(12):4165-4174. doi: 10.1021/acssynbio.4c00627. Epub 2024 Nov 28. 10.1021/acssynbio.4c00627 PubMed 39610115