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Addgene

PILAR
(Plasmid #22851)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR 2.1-TOPO
  • Backbone size w/o insert (bp) 3900
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PILAR
  • Alt name
    CLEC2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    910
  • Entrez Gene
    CLEC2A (a.k.a. INPE5792, KACL, PILAR, UNQ5792)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer M13-Reverse
  • 3′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PILAR was a gift from Jose Conejo-Garcia (Addgene plasmid # 22851 ; http://n2t.net/addgene:22851 ; RRID:Addgene_22851)

  • For your References section:

    PILAR is a novel modulator of human T-cell expansion. Huarte E, Cubillos-Ruiz JR, Nesbeth YC, Scarlett UK, Martinez DG, Engle XA, Rigby WF, Pioli PA, Guyre PM, Conejo-Garcia JR. Blood. 2008 Aug 15. 112(4):1259-68. 10.1182/blood-2007-12-130773 PubMed 18550855
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