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SUMO-PNGaseF
(Plasmid #228498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228498 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5630
  • Total vector size (bp) 6554
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ngl
  • Alt name
    PNGase F
  • Alt name
    Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F
  • Species
    Elizabethkingia miricola
  • Insert Size (bp)
    942
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • 10xHis-SUMO(Smt3) (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bacterial expression of 10xHis-SUMO tagged PNGase F. Expression of active enzyme is possible in Shuffle Cells

Kovács N, Farsang R, Szigeti M, Vonderviszt F, Jankovics H. Enhanced Recombinant Protein Production of Soluble, Highly Active and Immobilizable PNGase F. Mol Biotechnol. 2022 Aug;64(8):914-918. doi: 10.1007/s12033-022-00464-6. Epub 2022 Mar 4. PMID: 35244857; PMCID: PMC9259526.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SUMO-PNGaseF was a gift from Richard Baker (Addgene plasmid # 228498 ; http://n2t.net/addgene:228498 ; RRID:Addgene_228498)
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