pHyperTEV60
(Plasmid #228496)

• Purpose: 10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)
• Depositing Lab: Richard Baker
• Publication: Molecular Toolbox (unpublished)

Backbone

• Vector backbone: pET29b
• Backbone size w/o insert (bp): 5630
• Total vector size (bp): 6281
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TEV Protease
• Alt name: Tobacco Etch Virus Protease
• Species: Synthetic
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 10xHis-SUMO(Smt3) (N terminal on backbone)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • SUMO-HyperTEV60.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bacterial expression of 10xHis-SUMO tagged HyperTEV60

Sumida KH, Núñez-Franco R, Kalvet I, Pellock SJ, Wicky BIM, Milles LF, Dauparas J, Wang J, Kipnis Y, Jameson N, Kang A, De La Cruz J, Sankaran B, Bera AK, Jiménez-Osés G, Baker D. Improving Protein Expression, Stability, and Function with ProteinMPNN. J Am Chem Soc. 2024 Jan 24;146(3):2054-2061. doi: 10.1021/jacs.3c10941. Epub 2024 Jan 9. PMID: 38194293; PMCID: PMC10811672.