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Addgene

MZB206
(Plasmid #228493)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228493 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone size w/o insert (bp) 5387
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MSP2N2
  • Species
    Synthetic
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • 10xHis-SUMO(Smt3)-Avitag-HRV (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses HRV cleavable Avitagged MSP2N2 nanodiscs. Derived from Addgene plasmid: 29520. To be co-expressed with BirA to achieve biotinylation during expression.

Grinkova YV, Denisov IG, Sligar SG. Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers. Protein Eng Des Sel. 2010 Nov;23(11):843-8. doi: 10.1093/protein/gzq060. Epub 2010 Sep 3. PMID: 20817758; PMCID: PMC2953958.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MZB206 was a gift from Richard Baker (Addgene plasmid # 228493 ; http://n2t.net/addgene:228493 ; RRID:Addgene_228493)
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