MZB209
(Plasmid
#228492)
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PurposeBirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228492 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYCDuet-1
- Backbone size w/o insert (bp) 3710
- Total vector size (bp) 4673
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBirA
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Alt nameBifunctional ligase/repressor BirA
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Insert Size (bp)963
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Entrez GenebirA (a.k.a. b3973, ECK3965, bioR, dhbB)
- Promoter T7 Promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBrian Strahl Laboratory
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BirA co-expression vector (pACYC derived) for co-translational expression of biotinylation during bacterial expression with Avi-tagged constructs
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MZB209 was a gift from Richard Baker (Addgene plasmid # 228492 ; http://n2t.net/addgene:228492 ; RRID:Addgene_228492)
