MZB126
(Plasmid
#228482)
-
Purpose(Empty Backbone) "Empty" HA-secretion (membrane) vector for easy DNA Assembly (Mammalian cell Expression)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 228482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepαH
- Backbone size (bp) 4566
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Cloning Information
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
"Empty" vector for rapid cloning via DNA assembly methods (i.e. Gibson, Hifi, In-fusion, etc.) achieved via linearization by SmaI restriction enzyme cleavage. Encoded a hemagglutinin (HA) leader sequence for secretion to the plasma membrane
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MZB126 was a gift from Richard Baker (Addgene plasmid # 228482 ; http://n2t.net/addgene:228482 ; RRID:Addgene_228482)