MZB116
(Plasmid
#228480)
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Purpose(Empty Backbone) "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
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| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET29b
- Backbone size (bp) 5225
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Vector typeBacterial Expression
- Promoter T7 Promoter
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Tag
/ Fusion Protein
- CPD-10xHis (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector encodes for a portion of the Vibrio cholerae MARTX (rtxA) toxin. A majority of the pathogenic portions of the toxin are not present, therefore regular caution should be exercised when expressing the fusion protein.
"Empty" vector for rapid cloning via DNA Assembly (i.e. Gibson, HiFi, etc.) via SwaI linearization
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MZB116 was a gift from Richard Baker (Addgene plasmid # 228480 ; http://n2t.net/addgene:228480 ; RRID:Addgene_228480)
