Skip to main content
Addgene

pBAD-HaloGFP-Na2.4
(Plasmid #228470)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAD
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloGFP-Na2.4
  • Species
    Synthetic
  • Insert Size (bp)
    1619

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Hind3 (unknown if destroyed)
  • 5′ sequencing primer CTGTTTCTCCATACCCGTTTTTTGGGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-HaloGFP-Na2.4 was a gift from Robert Campbell (Addgene plasmid # 228470 ; http://n2t.net/addgene:228470 ; RRID:Addgene_228470)

  • For your References section:

    A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ions. Takeuchi S, Imai S, Terai T, Campbell RE. RSC Chem Biol. 2024 Dec 3. doi: 10.1039/d4cb00256c. 10.1039/d4cb00256c PubMed 39678364
Related items: