v5 Cas9-eVLP
(Plasmid
#228468)
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PurposeExpresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3-like
- Total vector size (bp) 11365
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMMLVgag(C507V)–Cas9
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SpeciesSynthetic
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Insert Size (bp)6609
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MutationMMLVgag(C507V)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 promoter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypCMV-MMLVgag-3xNES-Cas9 (Plasmid #181752)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
v5 Cas9-eVLP was a gift from David Liu (Addgene plasmid # 228468 ; http://n2t.net/addgene:228468 ; RRID:Addgene_228468)
