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PurposeExpresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228466 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Total vector size (bp) 9326
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMMLVgag-pro-pol(Q226P)
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SpeciesSynthetic; Murine leukemia virus
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Insert Size (bp)5307
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MutationQ226P
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer T3 promoter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypBS-CMV-gagpol (Plasmid #35614)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
v5 eVLP gag-pro-pol was a gift from David Liu (Addgene plasmid # 228466 ; http://n2t.net/addgene:228466 ; RRID:Addgene_228466)