v5 eVLP gag-pro-pol
(Plasmid #228466)

• Purpose: Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs
• Depositing Lab: David Liu
• Publication: Directed evolution of engineered virus-like particles with improved production and transduction efficiencies (unpublished)

Backbone

• Vector backbone: pBluescript
• Total vector size (bp): 9326
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MMLVgag-pro-pol(Q226P)
• Species: Synthetic; Murine leukemia virus
• Insert Size (bp): 5307
• Mutation: Q226P
• Promoter: CMV

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: T3 promoter

Resource Information

• Supplemental Documents:
  • v5 eVLP gag-pro-pol (Addgene 228466).gb
• A portion of this plasmid was derived from a plasmid made by: pBS-CMV-gagpol (Plasmid #35614)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry