pJRTCLCrVB1.3
(Plasmid #22844)

• Depositing Lab: Niki Robertson
• Publication: Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Backbone

• Vector backbone: pBlueScript II sk+(original backbone)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Vector type: Bacterial Expression ; cotton VIGS vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B DNA
• Alt name: pJRTB1.3 or pJRTCLCrVB1.3
• Alt name: CLCrVB1.3
• Alt name: JRTB1.3
• Species: Cotton Leaf Crumple Virus
• Insert Size (bp): 2425
• Mutation: 1.3 unit B componet length inserted into pBlueScript II Sk+. Cotton Leaf Crumple Virus BL1 and BR1 genes flanked by 2 common regions.
• GenBank ID: EU541443

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The wild-type CLCrV B component was provided by Dr. Judith K. Brown and Dr. Ali M. Idris from the University of Arizona, Tucson.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry