pJRTCLCrVA009
(Plasmid #22842)

• Depositing Lab: Niki Robertson
• Publication: Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Backbone

• Vector backbone: pBluescript SK+ II (original backbone)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Vector type: Bacterial Expression ; cotton VIGS vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: A DNA containing CHLI fragment
• Alt name: pJRT009/pJRTCLCrVA.009
• Alt name: CLCrVA:ChlI
• Alt name: JRT009
• Species: Cotton Leaf Crumple Virus
• Insert Size (bp): 2932
• Mutation: Cotton Leaf Crumple Virus Coat Protein (AR1 gene) was removed and replaced with a multiple cloning site. A 500-bp fragment of Magnesium Chelatase from Gossypium hirsutum was inserted.
• GenBank ID: EU541444

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HincII (destroyed during cloning)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The wild-type CLCrV A component was provided by Dr. Judith K. Brown and Dr. Ali M. Idris from the University of Arizona, Tucson.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are two point mutations with the author's sequence, which have been deemed non-critical by the depositing lab. One of the pt mutations lies outside the pop-out, the other is in
the Chli fragment.

--Must be bombarded with appropriate B DNA for systemic infection