pCCG_nit9fl_random_linker
(Plasmid
#228418)
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PurposeExpression of NIT-9 with moco resulfurase linkage region in N. crassa at the his-3 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePMF272
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Vector typeProtein expression in N. crassa
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNIT-9
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SpeciesH. sapiens (human)
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Mutationremoval of the linkage region and insertion of the moco resufurase linkage region
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Entrez GeneGPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCCG_nit9fl_random_linker was a gift from Robert Haensch (Addgene plasmid # 228418 ; http://n2t.net/addgene:228418 ; RRID:Addgene_228418)