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pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro
(Plasmid #228401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 11651
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SREBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3357
  • Mutation
    deleted N terminal activation domain (amino acids 1-29)
  • Entrez Gene
    SREBF1 (a.k.a. HMD, IFAP2, SREBP1, bHLHd1)
  • Promoter EF1a
  • Tag / Fusion Protein
    • mNeonGreen2x (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro was a gift from Dirk Daelemans (Addgene plasmid # 228401 ; http://n2t.net/addgene:228401 ; RRID:Addgene_228401)

  • For your References section:

    Ibetazol, a novel inhibitor of importin beta1-mediated nuclear import. Vercruysse T, Vanstreels E, Jacquemyn M, Boland S, Kilonda A, Allasia S, Vandecaetsbeek I, Klaassen H, Versele M, Chaltin P, Marchand A, Daelemans D. Commun Biol. 2024 Nov 23;7(1):1560. doi: 10.1038/s42003-024-07237-8. 10.1038/s42003-024-07237-8 PubMed 39580542
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