pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro
(Plasmid
#228398)
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PurposeEncodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4
- Total vector size (bp) 5444
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNLS/cMyc-AcGFP2x-NES/iKB2
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SpeciesSynthetic
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Insert Size (bp)1554
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer CGATAGTACTAACATACGCTCTCCA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro was a gift from Dirk Daelemans (Addgene plasmid # 228398 ; http://n2t.net/addgene:228398 ; RRID:Addgene_228398) -
For your References section:
Ibetazol, a novel inhibitor of importin beta1-mediated nuclear import. Vercruysse T, Vanstreels E, Jacquemyn M, Boland S, Kilonda A, Allasia S, Vandecaetsbeek I, Klaassen H, Versele M, Chaltin P, Marchand A, Daelemans D. Commun Biol. 2024 Nov 23;7(1):1560. doi: 10.1038/s42003-024-07237-8. 10.1038/s42003-024-07237-8 PubMed 39580542