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Addgene

pOPINF AtMKK5
(Plasmid #228396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPINF
  • Backbone manufacturer
    Berrow et al. (2007) PMID 17317681
  • Backbone size w/o insert (bp) 5196
  • Total vector size (bp) 6243
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MKK5
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1047
  • Entrez Gene
    MKK5 (a.k.a. AT3G21220, ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5, ATMAP2K_ALPHA, ATMEK5, ATMKK5, MAP KINASE KINASE 5, MAP kinase kinase 5, MAP2K_A, MEK5)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.25.558804 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINF AtMKK5 was a gift from Lennart Wirthmueller (Addgene plasmid # 228396 ; http://n2t.net/addgene:228396 ; RRID:Addgene_228396)

  • For your References section:

    Untargeted proteomics identifies plant substrates of the bacterial-derived ADP-ribosyltransferase AvrRpm1. Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, Susanne Matschi, eIvan Matić, and Lennart Wirthmueller. bioRxiv 2023.09.25.558804 10.1101/2023.09.25.558804
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