pDifferentiation
(Plasmid
#228393)
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Purposeintended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithography
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB3K3
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepLambda-luxR+LVAtag__pLux-mRuby2
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDifferentiation was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 228393 ; http://n2t.net/addgene:228393 ; RRID:Addgene_228393) -
For your References section:
Optogenetic patterning generates multi-strain biofilms with spatially distributed antibiotic resistance. Jin X, Riedel-Kruse IH. Nat Commun. 2024 Nov 1;15(1):9443. doi: 10.1038/s41467-024-53546-1. 10.1038/s41467-024-53546-1 PubMed 39487123