Lmir_cytGluRS_TP_pK7fWG2_IVD_FP611
(Plasmid
#228385)
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PurposeExpresses N-term transit peptide from Lophophytum mirabile cytoplasmic Glutamate aminoacyl-tRNA synthetase (TRINITY_DN2194_c0_g1_i3); determination of L. mirabile cytoplasmic GluRS target localization
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepK7fWG2_IVDFP611
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN-terminal transit peptide from L. mirabile cytoplasmic GluRS (TRINITY_DN2194_c0_g1_i3)
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SpeciesLophophytum mirabile
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Tag
/ Fusion Protein
- Green Florescent Protien (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lmir_cytGluRS_TP_pK7fWG2_IVD_FP611 was a gift from Daniel Sloan (Addgene plasmid # 228385 ; http://n2t.net/addgene:228385 ; RRID:Addgene_228385)