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Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611
(Plasmid #228374)

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Item Catalog # Description Quantity Price (USD)
Plasmid 228374 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pK7fWG2_IVD_FP616
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
  • Species
    Sapria himalayana
  • Tag / Fusion Protein
    • Green Florescent Protien (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611 was a gift from Daniel Sloan (Addgene plasmid # 228374 ; http://n2t.net/addgene:228374 ; RRID:Addgene_228374)

  • For your References section:

    Photosynthetic demands on translational machinery drive retention of redundant tRNA metabolism in plant organelles. DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB. Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 10.1073/pnas.2421485121. Epub 2024 Dec 18. 10.1073/pnas.2421485121 PubMed 39693336
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