Athal_PheRS_TP_pK7fWG2_IVD_FP611
(Plasmid
#228370)
-
PurposeExpresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 228370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
* Log in to view industry pricing.
Backbone
-
Vector backbonepK7fWG2_IVD_FP613
-
Vector typePlant Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameN-terminal transit peptide from Arabidopsis PheRS At3g58140 (Warren et al., 2023) with demonstrated dual mito/plastid targeting.
-
SpeciesA. thaliana (mustard weed)
-
Tag
/ Fusion Protein
- Green Florescent Protien (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Athal_PheRS_TP_pK7fWG2_IVD_FP611 was a gift from Daniel Sloan (Addgene plasmid # 228370 ; http://n2t.net/addgene:228370 ; RRID:Addgene_228370) -
For your References section:
Photosynthetic demands on translational machinery drive retention of redundant tRNA metabolism in plant organelles. DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB. Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 10.1073/pnas.2421485121. Epub 2024 Dec 18. 10.1073/pnas.2421485121 PubMed 39693336