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pET-SisTnpB1–ωRNA
(Plasmid #228365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-30a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SisTnpB1
  • Species
    Sulfolobus islandicus REY15A
  • GenBank ID
    SIRE_RS03225
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer ATatgCATCACCATCACCACCATCATCACtggagagccaaggagaagaat
  • 3′ sequencing primer TTAGCGGCCGCAAGCTTGTCGACgctataacac
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-SisTnpB1–ωRNA was a gift from Tao Liu (Addgene plasmid # 228365 ; http://n2t.net/addgene:228365 ; RRID:Addgene_228365)

  • For your References section:

    Collateral nuclease activity of TnpB triggered by high temperature enables fast and sensitive nucleic acid detection. Xu Y, Yin W, Cheng Y, Zeng W, Li W, Chen W, Wang F, Peng N, Ma L, Liu T. Commun Biol. 2024 Nov 20;7(1):1541. doi: 10.1038/s42003-024-07123-3. 10.1038/s42003-024-07123-3 PubMed 39567725
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