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Addgene

pMB1: All in one SpCas9 expression and sgRNA cloning backbone
(Plasmid #228363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size (bp) 13557
  • Modifications to backbone
    Expression of EF1A-SpCas9-2A-Puro-2A-mCherry
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR ; SpCas9 nuclease and spCas9 gRNA expression vector
  • Promoter mU6
  • Selectable markers
    Puromycin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB1: All in one SpCas9 expression and sgRNA cloning backbone was a gift from Michael Boettcher (Addgene plasmid # 228363 ; http://n2t.net/addgene:228363 ; RRID:Addgene_228363)
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