783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone
(Plasmid
#228362)
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Purpose(Empty Backbone) mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 8768
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Vector typeMammalian Expression, Lentiviral, CRISPR ; RfxCas13d gRNA expression vector
- Promoter mU6 and hU6
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Selectable markersPuromycin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone was a gift from Michael Boettcher (Addgene plasmid # 228362 ; http://n2t.net/addgene:228362 ; RRID:Addgene_228362)
Map uploaded by the depositor.
