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pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP
(Plasmid #228359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228359 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXR001: EF1a-CasRx-2A-EGFP
  • Total vector size (bp) 14341
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RfxCas13d
  • Alt name
    CasRx
  • Species
    H. sapiens (human); Ruminococcus flavefaciens
  • Insert Size (bp)
    3000
  • Promoter EF1A
  • Tags / Fusion Proteins
    • 2A-EGFP (C terminal on insert)
    • 2A-mCD4 (C terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGATGTTCCAGATTACGCTGGATCCGGCGCAACAAACTTCTCTCTGCTGAAACAAGCCGGAGATGTCGAAGAGAATCCTGGACCGCCGGACATGTGCCGAGC
  • 3′ sequencing primer GCCCTCTCCACTGCCGCCCTGGCGCTGTTGGTGCCGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mCD4 gene was amplified from the S. aureus Cas9 nuclease vector (Addgene #105998). pXR001 vector (Addgene #109049) was obtained from Addgene.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP was a gift from Michael Boettcher (Addgene plasmid # 228359 ; http://n2t.net/addgene:228359 ; RRID:Addgene_228359)
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