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Addgene

pYI2206
(Plasmid #228348)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phlTA mutant
  • Species
    Synthetic; Pseudomonas protegens
  • Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
  • Promoter KpGAPDH promoter
  • Tag / Fusion Protein
    • Three copies of VP16 (VP48)-Nuclear localization signal (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYI2206 was a gift from Jun Ishii (Addgene plasmid # 228348 ; http://n2t.net/addgene:228348 ; RRID:Addgene_228348)

  • For your References section:

    Designing strong inducible synthetic promoters in yeasts. Tominaga M, Shima Y, Nozaki K, Ito Y, Someda M, Shoya Y, Hashii N, Obata C, Matsumoto-Kitano M, Suematsu K, Matsukawa T, Hosoya K, Hashiba N, Kondo A, Ishii J. Nat Commun. 2024 Dec 19;15(1):10653. doi: 10.1038/s41467-024-54865-z. 10.1038/s41467-024-54865-z PubMed 39702268
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