pMT2096
(Plasmid
#228346)
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Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic; Aequorea victoria
- Promoter 2,4-Diacetylphloroglucinol-regulatable synthetic promoter (KpPhl1.1)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT2096 was a gift from Jun Ishii (Addgene plasmid # 228346 ; http://n2t.net/addgene:228346 ; RRID:Addgene_228346) -
For your References section:
Designing strong inducible synthetic promoters in yeasts. Tominaga M, Shima Y, Nozaki K, Ito Y, Someda M, Shoya Y, Hashii N, Obata C, Matsumoto-Kitano M, Suematsu K, Matsukawa T, Hosoya K, Hashiba N, Kondo A, Ishii J. Nat Commun. 2024 Dec 19;15(1):10653. doi: 10.1038/s41467-024-54865-z. 10.1038/s41467-024-54865-z PubMed 39702268