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Addgene

pMT1821
(Plasmid #228327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCfB2225
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rtetTA mutant
  • Species
    Synthetic; Escherichia coli
  • Mutation
    K8N
  • Promoter ScPGK1 promoter

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT1821 was a gift from Jun Ishii (Addgene plasmid # 228327 ; http://n2t.net/addgene:228327 ; RRID:Addgene_228327)

  • For your References section:

    Designing strong inducible synthetic promoters in yeasts. Tominaga M, Shima Y, Nozaki K, Ito Y, Someda M, Shoya Y, Hashii N, Obata C, Matsumoto-Kitano M, Suematsu K, Matsukawa T, Hosoya K, Hashiba N, Kondo A, Ishii J. Nat Commun. 2024 Dec 19;15(1):10653. doi: 10.1038/s41467-024-54865-z. 10.1038/s41467-024-54865-z PubMed 39702268
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