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Addgene

pMT1219
(Plasmid #228301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His
  • Species
    Synthetic; Severe acute respiratory syndrome-related coronavirus 2
  • Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter 2,4-Diacetylphloroglucinol-regulatable synthetic promoter (KpPhl6.0)
  • Tags / Fusion Proteins
    • MF_ secretion signal L42S/V50A mutant, MF_(2_Adv) (N terminal on insert)
    • GGG linker followed by His6 (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT1219 was a gift from Jun Ishii (Addgene plasmid # 228301 ; http://n2t.net/addgene:228301 ; RRID:Addgene_228301)

  • For your References section:

    Designing strong inducible synthetic promoters in yeasts. Tominaga M, Shima Y, Nozaki K, Ito Y, Someda M, Shoya Y, Hashii N, Obata C, Matsumoto-Kitano M, Suematsu K, Matsukawa T, Hosoya K, Hashiba N, Kondo A, Ishii J. Nat Commun. 2024 Dec 19;15(1):10653. doi: 10.1038/s41467-024-54865-z. 10.1038/s41467-024-54865-z PubMed 39702268
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