SJZ9-amdS
(Plasmid
#228261)
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PurposeTemplate plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSJZ9
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameamdS
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SpeciesAspergillus nidulans
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Insert Size (bp)2447
- Promoter ENO2 S.c.
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SJZ9-amdS was a gift from Zhiping Xie (Addgene plasmid # 228261 ; http://n2t.net/addgene:228261 ; RRID:Addgene_228261) -
For your References section:
Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae. Feng Y-H, Song J-Z, Zhu J, Xie Z. Microbiol Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24. 10.1128/spectrum.01320-24 PubMed 39387598