SJZ2-TRP1
(Plasmid
#228256)
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PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSJZ2
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRP1
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SpeciesKluyveromyces lactis
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Insert Size (bp)1433
- Promoter BBC1 S.c.
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SJZ2-TRP1 was a gift from Zhiping Xie (Addgene plasmid # 228256 ; http://n2t.net/addgene:228256 ; RRID:Addgene_228256) -
For your References section:
Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae. Feng Y-H, Song J-Z, Zhu J, Xie Z. Microbiol Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24. 10.1128/spectrum.01320-24 PubMed 39387598