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pLenti-CMV-GSDMD-eGFP-SV40p-BlasR
(Plasmid #228254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti- V6.3 Ultra-Chili
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSDMD
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_024736.7
  • Entrez Gene
    GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-GSDMD-eGFP-SV40p-BlasR was a gift from Judy Lieberman (Addgene plasmid # 228254 ; http://n2t.net/addgene:228254 ; RRID:Addgene_228254)

  • For your References section:

    Small-molecule GSDMD agonism in tumors stimulates antitumor immunity without toxicity. Fontana P, Du G, Zhang Y, Zhang H, Vora SM, Hu JJ, Shi M, Tufan AB, Healy LB, Xia S, Lee DJ, Li Z, Baldominos P, Ru H, Luo HR, Agudo J, Lieberman J, Wu H. Cell. 2024 Sep 2:S0092-8674(24)00898-5. doi: 10.1016/j.cell.2024.08.007. 10.1016/j.cell.2024.08.007 PubMed 39243763
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