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Addgene

pHPHS927
(Plasmid #228238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YTK089
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Promoter none
  • Tag / Fusion Protein
    • PuroR

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Beta globin
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Promoter TRE3GV
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.25.605204 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHPHS927 was a gift from Arvind Rasi Subramaniam (Addgene plasmid # 228238 ; http://n2t.net/addgene:228238 ; RRID:Addgene_228238)

  • For your References section:

    Decoding RNA Metabolism by RNA-linked CRISPR Screening in Human Cells. Nugent PJ, Park H, Wladyka CL, Chen KY, Bynum C, Quarterman G, Hsieh AC, Subramaniam AR. bioRxiv [Preprint]. 2024 Jul 26:2024.07.25.605204. doi: 10.1101/2024.07.25.605204. 10.1101/2024.07.25.605204 PubMed 39091804
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