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pB-3xFlag-dCas13-mTET2CD
(Plasmid #228234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    dCas13
  • Promoter CAG

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    TET2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2601
  • Mutation
    CD (cysteine-rich, dioxygenase domain) truncation of mouse TET2 (contains aa's 1044-1921)
  • Entrez Gene
    Tet2 (a.k.a. Ayu17-449, E130014J05Rik, mKIAA1546)
  • Promoter CAG

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Liu, pCMV-dCas13-M3nls (Addgene plasmid #155366 ; http://n2t.net/addgene:155366 ; RRID:Addgene_155366); and Yue Xiong, pcDNA3-FLAG-mTET2 (Addgene plasmid #89735 ; http://n2t.net/addgene:89735 ; RRID:Addgene_89735).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-3xFlag-dCas13-mTET2CD was a gift from Chuan He (Addgene plasmid # 228234 ; http://n2t.net/addgene:228234 ; RRID:Addgene_228234)

  • For your References section:

    RNA m(5)C oxidation by TET2 regulates chromatin state and leukaemogenesis. Zou Z, Dou X, Li Y, Zhang Z, Wang J, Gao B, Xiao Y, Wang Y, Zhao L, Sun C, Liu Q, Yu X, Wang H, Hong J, Dai Q, Yang FC, Xu M, He C. Nature. 2024 Oct 2. doi: 10.1038/s41586-024-07969-x. 10.1038/s41586-024-07969-x PubMed 39358506
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