pGGA016
(Plasmid
#228231)
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PurposeLevel 0 for 5xOp (GreenGate A module)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5xOp
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Insert Size (bp)402
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer ATTTAGGTGACACTATAGA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byIan Moore Lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGA016 was a gift from Alexis Maizel (Addgene plasmid # 228231 ; http://n2t.net/addgene:228231 ; RRID:Addgene_228231) -
For your References section:
GreenGate 2.0: Backwards compatible addons for assembly of complex transcriptional units and their stacking with GreenGate. Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, Petrasiunaite V, Faerber N, Distel K, Maizel A. PLoS One. 2023 Sep 8;18(9):e0290097. doi: 10.1371/journal.pone.0290097. eCollection 2023. 10.1371/journal.pone.0290097 PubMed 37682951