pHPHS309
(Plasmid
#228230)
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Purpose(Empty Backbone) GFP dropout backbone for sgRNA library cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYTK90
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.25.605204 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHPHS309 was a gift from Arvind Rasi Subramaniam (Addgene plasmid # 228230 ; http://n2t.net/addgene:228230 ; RRID:Addgene_228230) -
For your References section:
Decoding RNA Metabolism by RNA-linked CRISPR Screening in Human Cells. Nugent PJ, Park H, Wladyka CL, Chen KY, Bynum C, Quarterman G, Hsieh AC, Subramaniam AR. bioRxiv [Preprint]. 2024 Jul 26:2024.07.25.605204. doi: 10.1101/2024.07.25.605204. 10.1101/2024.07.25.605204 PubMed 39091804