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Addgene

pTYB1-T4gp46
(Plasmid #228211)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228211 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTBY1
  • Backbone manufacturer
    New England BioLabs
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    46
  • Species
    Escherichia phage T4
  • Promoter T7
  • Tag / Fusion Protein
    • Sce VMA intein-chitin binding domain (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a T144I mutation in T4gp46. This mutation is not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTYB1-T4gp46 was a gift from Stephen Benkovic (Addgene plasmid # 228211 ; http://n2t.net/addgene:228211 ; RRID:Addgene_228211)
  • For your References section:

    Biochemical characterization of bacteriophage T4 Mre11-Rad50 complex. Herdendorf TJ, Albrecht DW, Benkovic SJ, Nelson SW. J Biol Chem. 2011 Jan 28;286(4):2382-92. doi: 10.1074/jbc.M110.178871. Epub 2010 Nov 15. 10.1074/jbc.M110.178871 PubMed 21081488