pSLC25A13-APEX2
(Plasmid
#228198)
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Purposeplasmid encoding human SLC25A13 with an APEX protein tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC25A13
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SpeciesH. sapiens (human)
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Entrez GeneSLC25A13 (a.k.a. ARALAR2, CITRIN, CTLN2, NICCD)
- Promoter M13
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Tag
/ Fusion Protein
- APEX2 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GTA AAA CGA CGG CCA GT (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe gene was generated using Addgene #132038.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLC25A13-APEX2 was a gift from Stephen Benkovic (Addgene plasmid # 228198 ; http://n2t.net/addgene:228198 ; RRID:Addgene_228198) -
For your References section:
Purinosomes spatially co-localize with mitochondrial transporters. Sha Z, Benkovic SJ. J Biol Chem. 2024 Aug 2;300(9):107620. doi: 10.1016/j.jbc.2024.107620. 10.1016/j.jbc.2024.107620 PubMed 39098527