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Addgene

pADSS-EGFP
(Plasmid #228197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4633
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADSS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1368
  • Entrez Gene
    ADSS1 (a.k.a. ADSSL1, MPD5)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pADSS-EGFP was a gift from Stephen Benkovic (Addgene plasmid # 228197 ; http://n2t.net/addgene:228197 ; RRID:Addgene_228197)

  • For your References section:

    Quantitative analysis of purine nucleotides indicates that purinosomes increase de novo purine biosynthesis. Zhao H, Chiaro CR, Zhang L, Smith PB, Chan CY, Pedley AM, Pugh RJ, French JB, Patterson AD, Benkovic SJ. J Biol Chem. 2015 Mar 13;290(11):6705-13. doi: 10.1074/jbc.M114.628701. Epub 2015 Jan 20. 10.1074/jbc.M114.628701 PubMed 25605736
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