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pMRX-IPU-GFP-Rab5
(Plasmid #228139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IPU
  • Backbone size w/o insert (bp) 6100
  • Total vector size (bp) 7510
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1410
  • GenBank ID
    BC004842
  • Entrez Gene
    Rab5a (a.k.a. 2410015H04Rik, nnyRab5a)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a 75bp deletion in the backbone and LTR. This deletion is not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IPU-GFP-Rab5 was a gift from Noboru Mizushima (Addgene plasmid # 228139 ; http://n2t.net/addgene:228139 ; RRID:Addgene_228139)

  • For your References section:

    Organelle landscape analysis using a multiparametric particle-based method. Kurikawa Y, Koyama-Honda I, Tamura N, Koike S, Mizushima N. PLoS Biol. 2024 Sep 17;22(9):e3002777. doi: 10.1371/journal.pbio.3002777. eCollection 2024 Sep. 10.1371/journal.pbio.3002777 PubMed 39288101
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