pMRX-IPU-mTagBFP2-Sec61B
(Plasmid
#228136)
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PurposeExpresses mTagBFP2-Sec61B in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMRX-IPU
- Backbone size w/o insert (bp) 6100
- Total vector size (bp) 7129
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSec61B
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SpeciesH. sapiens (human)
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Insert Size (bp)1029
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GenBank IDNM_006808.3
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Entrez GeneSEC61B
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Tag
/ Fusion Protein
- mTagBFP2 (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-IPU-mTagBFP2-Sec61B was a gift from Noboru Mizushima (Addgene plasmid # 228136 ; http://n2t.net/addgene:228136 ; RRID:Addgene_228136) -
For your References section:
Organelle landscape analysis using a multiparametric particle-based method. Kurikawa Y, Koyama-Honda I, Tamura N, Koike S, Mizushima N. PLoS Biol. 2024 Sep 17;22(9):e3002777. doi: 10.1371/journal.pbio.3002777. eCollection 2024 Sep. 10.1371/journal.pbio.3002777 PubMed 39288101