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Addgene

pAGK61
(Plasmid #228121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUGW-H1 (Addgene plasmid #25870)
  • Backbone size w/o insert (bp) 4363
  • Total vector size (bp) 6523
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2162
  • Promoter CAG for RT, U6/H1 for ncRNA/gRNA

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGK61 was a gift from Seth Shipman (Addgene plasmid # 228121 ; http://n2t.net/addgene:228121 ; RRID:Addgene_228121)

  • For your References section:

    An experimental census of retrons for DNA production and genome editing. Khan AG, Rojas-Montero M, Gonzalez-Delgado A, Lopez SC, Fang RF, Crawford KD, Shipman SL. Nat Biotechnol. 2024 Sep 17. doi: 10.1038/s41587-024-02384-z. 10.1038/s41587-024-02384-z PubMed 39289529
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